When Should I use this Test?
Hybrid purity is an important component to seed production. In some cases, Isoelectric Focusing (IEF) can replace the traditional grow-out; it can also complement the results obtained from the phenotypic assessment during grow-out analysis. IEF is a technique of separating total extracted seed protein to create a unique banding pattern. Hybrid corn will share bands in common with the male and female parents. IEF can be used to identify off-types and selfs.
What Technique is Used?
Seed tissue is crushed and extracted. The extracted protein is separated by pH on a polyacrylamide gel. A unique banding pattern is displayed once the gel is stained. Female and Male parents present unique banding patterns and parent-specific bands can be observed in true hybrid seed. Off-types or out-crosses are easily recognizable as foreign bands will be presented. This test is performed on 100 individual hybrid seeds. Parent seed is required for testing; female seed is required for scoring selfs; it is preferred that both male and female seed be submitted. Results are returned as a percent of hybrid seed with off-types and selfs noted.